Product Name: Seta-670-NHS
Product Number:
K8-1342 Target Group:
NH2Excitation Light Sources:
Medium 1:
PB 7.4λAb max. [nm]:
667ε [M–1 cm–1]:
180000λEm max. [nm]:
688QY [%]:
7FLT [ns]:
0.42Medium 2:
BSA, D/P=1.5λAb max. [nm]:
681ε [M–1 cm–1]:
180000λEm max. [nm]:
696QY [%]:
27FLT [ns]:
2.43Specs Sheet:
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Description
- High hydrophilic, amine-reactive fluorescent label containing one reactive NHS-ester group
Applications
- Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
- Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule
- Fluorescence Resonance Energy Transfer (FRET) applications
- Single Molecule Applications — Seta-670 shows extreme low blinking in single molecule measurements
- Flow Cytometry
- Immunofluorescence
- Gene Expression
- Homogeneous Assays
- Assessment of protein structure
Advantages
- Perfectly suited for excitation with the 380, 404, 635, 670-nm diode lasers, LEDs, and UV light
- Sensitive; high extinction coefficients and high quantum yields up to 50% after covalent attachment to proteins
- Quantum yield is highly increased after covalently attachment to proteins and other biomolecules
- pH-insensitive between pH 3 and pH 10
- Good aqueous solubility; this label does not alter the solubility of the protein conjugate
- High photostability; e.g. compared to fluorescein or Cy5TM
- Low molecular weight — Seta dyes do not add substantial mass to the conjugates
- Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides
