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SETA BioMedicals produces, commercializes and licenses proprietary fluorescent detection reagents (fluorescent dyes, probes, labels, tandems, and standards) for biomedical research applications.

These detection reagents can be ordered over the phone at (217) 417 2160, via e-mail at orders@setabiomedicals.com or with our online shopping cart and are delivered the next day in the continental USA and within days at any other destination around the world.

TECHNOLOGY HIGHLIGHTS

Highly Bright, Water-Soluble Rotaxane Labels
Novel, extremely bright and photostable probes and labels based on squaraine rotaxanes. These unique fluorescent reporters combine all desirable features of common fluorophores including high chemical and photochemical stability.

Photostability comparison of several red emitting dyes
Phycobiliprotein Tandem Conjugates
Bright and photostable tandem-conjugates of APC, PE and PerCP with Seta dyes for flow cytometry and immuno-fluorescence applications.

Flow cytometry measurment of blood cells stained with CD3 – Seta-APC-780
Labeling Technologies
Next generation labels for proteins, oligonucleotides and other biomolecules with exceptional brightness and photostability.

Mouse egg cell stained with
Square-655 and Square-460 Mouse egg cell stained with
Square-670-Carboxy
Click Chemistry Reagents
Azides and proprietary DBCO derivatives of long-lifetime and 2P-imaging dyes with 2PACS of several thousand GM for Cu-catalyzed and Cu-free click chemistry reactions
Viscosity-Sensitive Probes and Labels
Large Stokes' shift fluorescent molecular rotors with high sensitivity and a large dynamic range in lifetime and intensity mode
Exeptional Dyes for 2-Photon Microscopy
Fluorescent probes and labels with exceptionally high 2-photon cross sections close to 10,000 GM based on squaraines and squaraine rotaxanes

2-PCS comparison of red emitting Seta and SeTau dyes
Fluorescence Lifetime Labels
Fluorescence lifetime labels with lifetimes from 0.5 to 32 ns for fluorescence lifetime and polarization applications
Fluorescence Standards
Fluorescence standards are utilized in the calibration and validation of fluorescence instruments, luminescence readers, and as fluorescence reference materials. The standard kits offered by SETA are ready and easy to use!

APPLICATIONS

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In a comparative study of 13 dyes in Nature Chem. Biol., SeTau-647 (K9-4149 and K9-4148), a squaraine rotaxane dye, exhibited the best photobleaching performance in a single molecule application with an exponential lifetime of 105.8 ns (twice as long as the 2^nd best dye Atto-647N).

Single molecule study of 13 fluorescent imaging dyes
Fluorescence Imaging

Squaraine rotaxane dye SeTau-647 outperforms Alexa dyes (Alexa 488, Alexa 568) in signal-to-background ratio and in vivo imaging properties.

Signal-to-background for SeTau-647 and Alexa 488 in imaging applications
2-Photon Microscopy

Compared to conventional cyanine dyes, squaraine and squaraine-rotaxanes exhibit 10 – 50-times higher 2-photon cross-sections in the order of several thousand GM.

Z-projection of a neuron filled with a squaraine rotaxane-labeled peptide
Superresolution Microscopy

Seta and SeTau dyes are a class of extremely bright and photostable dyes for fluorescence imaging and are also well suited for STED and STORM applications.

STED image of HeLa-M cell nuclei labeled with primary mAB414 antibody and a secondary antibody labeled with SeTau-647
DNA and Protein Microarrays

Our highly fluorescent squaraine rotaxanes exhibit high chemical stability in particular against oxidative reagents such as peroxides or ozone and therefore are excellent detection reagents for DNA and protein microarrays where the fluorescence signals of cyanine dyes such as Cy5 and Alexa 647 are strongly dependent on the concentration of ozone during post-hybridization array washing.


Mouse egg cell stained with Square-655 and Square-460	Mouse egg cell stained with Square-670-Carboxy