Reactive Labels

Square, Seta, and SeTau dyes are a series of dyes that span the whole visible spectrum and the NIR. They are available with various functionalities: carboxylic acids, as amine-reactive NHS esters for labeling of amino groups on peptides, proteins or amino-modified DNA, as maleimides for coupling to thiol-groups in proteins and thiol-modified oligos and as amino-modified labels (on request).

With a few exceptions all these reagents are available in either 1 mg or 5 mg pack sizes. Please do inquire about the availability of bulk quantities.

			Excitation Light Sources												Characteristics						Characteristics
Product Number (Specs Sheet)	Product Name (Product Info)	Target Group	380	405	436	488	532	594	635	650	680	700	750	780	Medium	λ abs [nm]	ε [M –1 cm–1]	λ em [nm]	QY [%]	FLT [ns]	Medium	λ abs [nm]	ε [M–1 cm–1]	λ em [nm]	QY [%]	FLT [ns]	Buy
K1-204 K1-204	SeTau-380-NHS SeTau-380-NHS	NH2	•	•											Water	270	23,800	480	56	32.5	BSA, D/P=1	383	23,800	468	44	30.6
K5-3222 K5-3222	Seta-580-NHS Seta-580-NHS	NH2					•								PB 7.4	578	90,000	602	55		BSA, D/P=1	585		609	15
K7-547 K7-547	SeTau-405-NHS SeTau-405-NHS	NH2	•	•											PB 7.4	405	13,800	518	80	9.3	IgG, D/P=8	405	13,800	518	19	8.4
K7-567 K7-567	SeTau-405-Azide SeTau-405-Azide	triple-CC	•	•											PB 7.4	405	13,800	518	80	9.3	EtOH	391	15,000	498	55	8.5
K8-1342 K8-1342	Seta-670-NHS Seta-670-NHS	NH2	•	•					•	•					PB 7.4	667	180,000	688	7	0.42	BSA, D/P=1.5	681	180,000	696	27	2.43
K7-545 K7-545	SeTau-425-NHS SeTau-425-NHS	NH2	•	•	•										PB 7.4	425	4,200	545	39	26.2	BSA, D/P=1	427	4,200	528	16	26.2
K8-1346 K8-1346	Seta-670-Azide Seta-670-Azide	triple-CC	•	•					•	•					PB 7.4	667	180,000	686	7		PB 7.4	667	180,000	686	7
K8-1352 K8-1352	Square-660-NHS Square-660-NHS	NH2	•	•					•	•					PB 7.4	657	182,000	676	3	0.27	BSA D/P=1.2	676	182,000	695	13	3.32
K8-1357 K8-1357	Square-680-NHS Square-680-NHS	NH2	•	•					•	•					MeOH	668	208,000	690	10		CHCl3	683	202,000	701	47
K8-1367 K8-1367	Seta-670-pH-di-NHS Seta-670-pH-di-NHS	NH2	•	•					•	•	•				PB 6.0	672	93,000	694	19		IgG, D/P=1	684		703	7
K8-1384 K8-1384	Seta-700-NHS Seta-700-NHS	NH2	•	•						•	•	•			PB 7.4	687	177,000	703	6		IgG D/P=1	705	177,000	715	4
K8-1407 K8-1407	Square-650-pH-NHS Square-650-pH-NHS	NH2						•	•	•					PB 5.6	653	135,000	671	16	1.17	IgG, D/P=0.8 pH=2.0	662	135,000	677	7	1.52
K8-1641 K8-1641	Seta-632-Maleimide Seta-632-Maleimide	SH							•						PB 7.4	633	270,000	642	5
K8-1642 K8-1642	Seta-632-NHS Seta-632-NHS	NH2							•						PB 7.4	632	280,000	641	6		IgG, D/P=1	637		647	18
K8-1649 K8-1649	SQ-740-NHS SQ-740-NHS	NH2								•	•	•	•		PB 7.4	740	44,000		0	0
K8-1663 K8-1663	Seta-633-NHS Seta-633-NHS	NH2						•	•						PB 7.4	633	250,000	644	7	0.25	IgG D/P=1	637	250,000	647	26	1.49
K8-1667 K8-1667	Seta-635-pH-di-NHS Seta-635-pH-di-NHS	NH2							•						PB 7.4	640	188,000	656	33	1.8	IgG, D/P=1	646		663	21
K8-1672 K8-1672	Seta-646-NHS Seta-646-NHS	NH2							•						PB 7.4	646	207,000	656	10	0.38	IgG, D/P=1	650	207,000	661	33
K8-1682 K8-1682	Seta-660-di-NHS Seta-660-di-NHS	NH2							•	•					PB 7.4	661	220,000	672	11	0.9	IgG D/P=1	665	220,000	675	49
K8-1696 K8-1696	Seta-633-Azide Seta-633-Azide	triple-CC						•	•						PB 7.4	633	250,000	644	7		PB 7.4	633	250,000	644	7
K8-1902 K8-1902	SQ-565-NHS SQ-565-NHS	NH2					•	•							Water	560	22,000		0	0	CHCl3	565
K8-1922 K8-1922	SQ-780-NHS SQ-780-NHS	NH2	•	•	•						•	•	•	•	MeOH	782	115,000		0	0	CHCl3	806			0	0
K8-3002 K8-3002	Seta-470-NHS Seta-470-NHS	NH2		•	•	•									water	469	50,000	521	1.9		BSA, D/P=1	469		515	28
K8-3335 K8-3335	Seta-555-NHS Seta-555-NHS	NH2					•								PB 7.4	555	155,000	570	7		IgG, D/P=1	556		571	13
K8-3345 K8-3345	Seta-555-DBCO Seta-555-DBCO	N3					•								PB 7.4	555	155,000	570	7
K8-3346 K8-3346	Seta-555-Azide Seta-555-Azide	triple-CC					•								PB 7.4	555	155,000	570	7
K8-5035 K8-5035	Seta-650-NHS Seta-650-NHS	NH2							•	•					PB 7.4	651	200,000	671	28		IgG, D/P=1	654		673	37
K8-5036 K8-5036	Seta-650-Maleimide Seta-650-Maleimide	SH							•	•					PB 7.4	652	200,000	672	28
K8-5045 K8-5045	Seta-650-DBCO Seta-650-DBCO	N3							•	•					PB 7.4	653	200,000	674	28
K8-5046 K8-5046	Seta-650-Azide Seta-650-Azide	triple-CC							•	•					PB 7.4	651	200,000	671	28
K8-5075 K8-5075	Seta-680-NHS Seta-680-NHS	NH2							•	•	•				PB 7.4	678	200,000	698	24		IgG, D/P=1	680		701	30
K8-7045 K8-7045	Seta-750-DBCO Seta-750-DBCO	N3									•	•	•		PB 7.4	754	234,000	782	21
K8-7046 K8-7046	Seta-750-Azide Seta-750-Azide	triple-CC									•	•	•		PB 7.4	750	200,000	779	22
K8-7055 K8-7055	Seta-780-NHS Seta-780-NHS	NH2										•	•	•	PB 7.4	777	215,000	807	1.2
K9-4119 K9-4119	SeTau-665-NHS SeTau-665-NHS	NH2								•	•	•			PB 7.4	664	160,000	712	53	3.1	IgG, D/P=1.5	662		716	46
K9-4142 K9-4142	SeTau-647-di-NHS SeTau-647-di-NHS	NH2							•	•					PB 7.4	650	200,000	694	65	3.2	IgG, D/P=0.5	649	200,000	691	58	3.2
K9-4148 K9-4148	SeTau-647-Maleimide SeTau-647-Maleimide	SH							•	•					PB 7.4	648	200,000	692	45	3.2	PB 7.4	648	200,000	692	45	3.2
K9-4149 K9-4149	SeTau-647-NHS SeTau-647-NHS	NH2							•	•					PB 7.4	649	200,000	695	61	3.2	IgG, D/P=1	648	200,000	694	59	3.3
K9-4169 K9-4169	SeTau-670-NHS SeTau-670-NHS	NH2							•	•	•				PB 7.4	673	275,000	694	36	1.6	IgG D/P = 4	672		693	14	1.75
K8-3402 K8-3402	Zy3-NHS Zy3-NHS	NH2					•								PB 7.4	549	150,000	564	4	0.3
K8-5402 K8-5402	Zy5-NHS Zy5-NHS	NH2							•						PB 7.4	647	250,000	665	27	1
K8-5422 K8-5422	Zy5.5-NHS Zy5.5-NHS	NH2							•	•	•				PB 7.4	676	190,000	694	23	1
K8-3403 K8-3403	Zy3-Maleimide Zy3-Maleimide	SH					•								PB 7.4	549	150,000	564	4	0.3
K8-5403 K8-5403	Zy5-Maleimide Zy5-Maleimide	SH							•						PB 7.4	647	250,000	665	27	1
K8-5423 K8-5423	Zy5.5-Maleimide Zy5.5-Maleimide	SH							•	•	•				PB 7.4	676	190,000	694	23	1
K8-1388 K8-1388	Seta-700-di-NHS Seta-700-di-NHS	NH2	•	•						•	•	•			PB 7.4	688	180,000	704	11		IgG D/P=1	696		713	23
K8-1341 K8-1341	Seta-670-Maleimide Seta-670-Maleimide	SH	•	•					•	•					PB 7.4	667	180,000	688	7
K8-1671 K8-1671	Seta-646-Maleimide Seta-646-Maleimide	SH							•						PB 7.4	647	210,000	657	8		IgG, D/P=1	650		661	31
K4-205 K4-205	Seta-375-NHS Seta-375-NHS	NH2	•												PB 7.4	372	24,500	476	86	3.1	IgG, D/P=5	372	24,500	480	42	3.5
K7-548 K7-548	SeTau-405-Maleimide SeTau-405-Maleimide	SH	•	•											PB 7.4	405	13,800	518	51	9.1	EtOH	391	15,000	498	32	8.5
K8-7522 K8-7522	Seta-750-NHS Seta-750-NHS	NH2									•	•	•		PB 7.4	753	230,000	780	14		IgG D/P=1	756		783	17
KS-650 KS-650	590–650 nm FLT Standard 590–650 nm FLT Standard								•						PB 7.4	647	250,000	665	27	1.05
K8-3003 K8-3003	Seta-470-Maleimide Seta-470-Maleimide	SH		•	•	•									Water	469	50,000	521	1.9		EtOH	462	50,000	515	2.2
K8-7402 K8-7402	Zy7-NHS Zy7-NHS	NH2									•	•	•		PB 7.4	749	200,000	774	13	0.57
DD1 DD1	DD1 2D DIGE Kit DD1 2D DIGE Kit	NH2					•		•
DD2 DD2	DD2 2D DIGE Kit DD2 2D DIGE Kit	NH2					•		•
DD3 DD3	DD3 2D DIGE Kit DD3 2D DIGE Kit	NH2					•		•
K7-544 K7-544	SeTau-425-Maleimide SeTau-425-Maleimide	SH	•	•	•										PB 7.4	425	4,200	545	39	26.2	PB 7.4	425	4,200	545	39	26.2
K4-225 K4-225	Seta-385-NHS Seta-385-NHS	NH2	•												PB 7.4	384	25,000	512	78		IgG, D/P=6	387	25,000	505	29
K8-1389 K8-1389	Seta-700-NHS Seta-700-NHS	NH2		•						•	•	•			PB 7.4	688	162,000	703	14		IgG D/P=1	694		710	18
K6-5052 K6-5052	Seta-405 NHS Seta-405 NHS	NH2	•	•											PB 7.4	401	30,000	452	70		IgG D/P=1	405		453	56
K4-215 K4-215	Seta-400-NHS Seta-400-NHS	NH2	•	•											PB 7.4	399	24,000	576	34		IgG D/P=1	401		569	19
K9-4179 K9-4179	SeTau-680-NHS SeTau-680-NHS	NH2							•	•	•				PB 7.4	683	295,000	705	58	2.9	IgG D/P=1	682		703	47	3.1
K9-4154 K9-4154	SeTau-647-DBCO SeTau-647-DBCO	N3							•	•					PB 7.4	649	200,000	690	60	3.2
K8-1662 K8-1662	Seta-640-di-NHS Seta-640-di-NHS	NH2							•						PB 7.4	636	181,000	646	13	0.7	IgG D/P=1	640		650	30
K9-3152 K9-3152	SeTau-488-NHS SeTau-488-NHS	NH2				•									PBS 7.4	486	59,000	532	27
K9-4159 K9-4159 new	SeTau-660-NHS SeTau-660-NHS	NH2								•					PB 7.4	663	240,000	694	50	3.36	IgG Conjugate D/P = 1	663	240,000	694	56

 

Labeling of Amino-groups:

The following functional groups available for labeling of amino-groups in proteins or amino-modified oligos:

Among all these functionalities N-hydroxysuccinimide (NHS) ester are the preferred reactive functionality for labeling of amino-groups due to the formation of highly stable amide bonds with amines. Sulfonyl chlorides also form stable sulfamide bonds but the reaction also generates HCl, which is a not so desirable by-product. In addition sulfonyl chlorides are more difficult to synthesize.

 

Labelling of Thiol-groups:

Maleimides are the preferred functionality for labeling of small molecules such as peptide and oligo-nucleotides. Maleimides form bonds with thiols at relatively mild conditions and with reasonable yields. Maleimides are less susceptible to hydrolysis in comparison with NHS esters.

We would like to refer you to our labeling procedures for specific guidance on how to label with NHS-esters or maleimides.

 

Azides:

Several of our most sensitive Seta Dyes are now available as azides to be reacted with C≡C-triple bonds in either a Cu(I)-catalized or Cu-free 1,3-dipolar cycloaddition reaction to a triazole (see figure). Both reactions proceed in aqueous environments with adequate efficiency.

Cu-catalyzed cycloaddition:

 

Cyclo-Octyne (DBCO) Derivatives:

Strain-mediated cyclization reactions of cyclo-octynes proceed without the need for a Cu-catalyst. The metal-free reaction has wider applicability and is compatible with cell-based applications.

Cu-free, strain-mediated cycloaddition: