Product Name: Seta-385-NHS
Product Number:
K4-225 Target Group:
NH2Excitation Light Sources:
Medium 1:
PB 7.4λAb max. [nm]:
384ε [M–1 cm–1]:
25000λEm max. [nm]:
512QY [%]:
78Medium 2:
IgG, D/P=6λAb max. [nm]:
387ε [M–1 cm–1]:
25000λEm max. [nm]:
505QY [%]:
29Specs Sheet:
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Description
- Hydrophilic, positively charged, amine-reactive label containing one NHS-ester group
Applications
- Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
- Resonance Energy Transfer (RET)
- Flow Cytometry
- Immunofluorescence
- Gene Expression
- Homogeneous Assays
- Assessment of protein structure
Advantages
- Perfectly suited for excitation with the 330 and 380-nm diode lasers and UV light
- Extremely large Stokes' shift of over 120 nm
- High extinction coefficients and high quantum yields of 78%
- Low non-specific binding
- pH-insensitive between pH 3 and pH 10
- Good aqueous solubility; this label does not alter the solubility of the protein conjugate
- High photostability; e.g. compared to fluorescein
- Low molecular weight — Seta dyes do not add substantial mass to the conjugates
- Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides
