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K5-3222
Product Name: Seta-580-NHS
Product Number:
K5-3222
Target Group:
NH
2
Excitation Light Sources:
Medium 1:
PB 7.4
λ
Ab
max. [nm]:
578
ε [M
–1
cm
–1
]:
90000
λ
Em
max. [nm]:
602
QY [%]:
55
Medium 2:
BSA, D/P=1
λ
Ab
max. [nm]:
585
λ
Em
max. [nm]:
609
QY [%]:
15
Specs Sheet:
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Description
Amine-reactive, neutral fluorescent label containing one reactive NHS-ester groups
Applications
Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
Advantages
Perfectly suited for excitation with the 532-nm diode laser
Sensitive; high extinction coefficient and high quantum yield up to 55% after covalent attachment to proteins
Good aqueous solubility; this label does not alter the solubility of the dye-conjugate
Low molecular weight —
K5-3222
does not add substantial mass to the conjugates
Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides