Product Name: Seta-700-di-NHS

Product Number:

K8-1388

Target Group:

NH₂

Excitation Light Sources:

Medium 1:

PB 7.4

λ_Ab max. [nm]:

688

ε [M^–1cm^–1]:

180000

λ_Em max. [nm]:

704

QY [%]:

Medium 2:

IgG D/P=1

λ_Ab max. [nm]:

696

λ_Em max. [nm]:

713

QY [%]:

Medium 3:

MeOH

λ_Ab max. [nm]:

701

ε [M^–1cm^–1]:

175000

λ_Em max. [nm]:

719

QY [%]:

Specs Sheet:

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Description

Bright, water soluble, amine-reactive fluorescent label with two reactive NHS-ester groups. Quantum yields up to 25% when labeled to IgG.

Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides.
Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule.
Resonance Energy Transfer (RET).
Flow Cytometry.
Immunofluorescence.
Gene Expression.
Homogeneous Assays.
Assessment of protein structure.

Perfectly suited for excitation with the 380, 405, 650, and 670-nm diode lasers, and UV light
Q.Y.s up to 25% upon covalent attachment to IgG
pH-insensitive between pH 4 and pH 9
Good aqueous solubility; this label does not alter the solubility of the protein conjugate
Higher photostability compared to fluorescein, Cy5^TM, Cy7^TM or Alexa Fluor^TM 700
Low molecular weight — Seta dyes do not add substantial mass to the conjugates
Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides