Product Name: Seta-700-NHS

Product Number:

K8-1389

Target Group:

NH₂

Excitation Light Sources:

Medium 1:

PB 7.4

λ_Ab max. [nm]:

688

ε [M^–1cm^–1]:

162000

λ_Em max. [nm]:

703

QY [%]:

Medium 2:

IgG D/P=1

λ_Ab max. [nm]:

694

λ_Em max. [nm]:

710

QY [%]:

Specs Sheet:

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Description

Water soluble, amine-reactive fluorescent label with one reactive NHS-ester groups.
High extinction coefficient and quantum yields up to 18% when labeled to IgG.

Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides.
Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule.
Resonance Energy Transfer (RET).
Flow Cytometry.
Immunofluorescence.
Gene Expression.
Homogeneous Assays.
Assessment of protein structure.

Perfectly suited for excitation with the 405, 670 or 690-nm diode lasers
Q.Y.s up to 18% upon covalent attachment to IgG
pH-insensitive between pH 4 and pH 9
Good aqueous solubility; this label does not alter the solubility of the protein conjugate
Higher photostability compared to fluorescein, Cy7^TM or Alexa Fluor^TM 700
Low molecular weight — Seta dyes do not add substantial mass to the conjugates
Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides