Product Name: Square-635-Maleimide
Product Number:
K8-1613 Target Group:
SHExcitation Light Sources:
Medium 1:
pH 7.4λAb max. [nm]:
636ε [M–1 cm–1]:
180000λEm max. [nm]:
649QY [%]:
8Specs Sheet:
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Description
- Thiol-reactive, pH-sensitive fluorescent label containing one reactive maleimide group.
Applications
- Covalent labeling of thiol-modified peptides, oligonucleotides, and proteins.
- Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule.
Advantages
- Perfectly suited for excitation with the 594-nm and 635-nm diode laser.
- Sensitive; high extinction coefficient and high quantum yield after covalent attachment to proteins.
- Low non-specific binding.
- pH-sensitive between pH 9 and pH 12, and pH-insensitive between pH 3 and pH 9.
- Good aqueous solubility; this label does not alter the solubility of the protein conjugate.
- High photostability; e.g. compared to CypHer 5TM.
- Low molecular weight — Squaredyes do not add substantial mass to the conjugates.
- Ideal for non-radioactive labeling of thiol-modified peptides, oligonucleotides, and proteins.
