Amine-reactive quencher containing one reactive NHS-ester group for labeling of proteins, peptides, molecular beacons, linear probes, and amino-modified oligonucleotides.
”Dark acceptor” for FRET-applications in combination with Seta-632, Seta-633, Seta-646, Seta-660, Seta-670, Seta-700, SeTau-647, Cy5TM, Cy5.5TM, Cy7TM, Alexa 647 TM, Alexa 660TM andAlexa 680TM as fluorescent donors.
Hybridization Assays.
Real-time PCR.
SNPs detection
Applications
”Dark acceptor” for FRET-applications in combination with Seta-632, Seta-633, Seta-646, Seta-660, Seta-670, Seta-700, SeTau-647, Cy5TM, Cy5.5TM, Cy7TM, Alexa 647 TM, Alexa 660TM andAlexa 680TM as fluorescent donors.
Hybridization Assays.
Real-time PCR.
SNPs detection
Advantages
pH-insensitive between pH 3 and pH 10
Water-soluble; label does not alter the solubility of bioconjugates.