Product Name: Seta-660-di-NHS

Product Number:

K8-1682

Target Group:

NH₂

Excitation Light Sources:

Medium 1:

PB 7.4

λ_Ab max. [nm]:

661

ε [M^–1cm^–1]:

220000

λ_Em max. [nm]:

672

QY [%]:

FLT [ns]:

0.9

Medium 2:

IgG D/P=1

λ_Ab max. [nm]:

665

ε [M^–1cm^–1]:

220000

λ_Em max. [nm]:

675

QY [%]:

Specs Sheet:

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Description

Extremely bright, hydrophilic, amine-reactive label containing two NHS-ester groups. QYs of up to 50% when covalently labeled to IgG.

Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
Fluorescence intensity and fluorescence polarization-based applications
Resonance Energy Transfer (RET)
Flow Cytometry
Immunofluorescence
Gene Expression
Homogeneous Assays
Microarrays

Perfectly suited for excitation with the 635-nm and 650-nm diode lasers
Sensitive; high extinction coefficients and high quantum yields after covalent attachment to biomolecules
Brighter replacement for Alexa 660 (QY = 37%, ε = 132,000 M^–1cm^–1). Seta-660: QY = 49%, ε = 220,000 M^–1cm^–1
pH-insensitive between pH 3 and pH 10
Good aqueous solubility; this label does not alter the solubility of the bioconjugate
High photostability; e.g. compared to fluorescein, Cy5 and Alexa 647 or Alexa 660
Low molecular weight — Seta dyes do not add substantial mass to the conjugates
Ideal for non-radioactive labeling of proteins, amino-modified oligonucleotides and amino-modified lipids