Product Name: Seta-650-Maleimide
Product Number:
K8-5036 Target Group:
SHExcitation Light Sources:
Medium 1:
PB 7.4λAb max. [nm]:
652ε [M–1 cm–1]:
200000λEm max. [nm]:
672QY [%]:
28Specs Sheet:
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Description
- Highly hydrophilic, thiol-reactive label containing one maleimide group.
Applications
- Covalent labeling of proteins, peptides, thiol-modified DNA and thiol-modified oligonucleotides
- Fluorescence intensity and fluorescence polarization-based applications
- Resonance Energy Transfer (RET)
- Flow Cytometry
- Immunofluorescence
- Gene Expression
- Homogeneous Assays
- Microarrays
Advantages
- Perfectly suited for excitation with the 635-nm and 650-nm diode lasers
- Sensitive; high extinction coefficients and high quantum yields after covalent attachment to biomolecules
- pH-insensitive between pH 3 and pH 10
- Good aqueous solubility; this label does not alter the solubility of the bioconjugate
- High photostability compared to fluorescein, Atto 647, DyLight 650, Alexa 647 or Cy5TM
- Low molecular weight — Seta dyes do not add substantial mass to the conjugates
- Ideal for labeling of proteins, and thiol-modified oligonucleotides, lipids and peptides
