Product Name: Seta-680-NHS
Product Number:
K8-5075 Target Group:
NH2Excitation Light Sources:
Medium 1:
PB 7.4λAb max. [nm]:
678ε [M–1 cm–1]:
200000λEm max. [nm]:
698QY [%]:
24Medium 2:
IgG, D/P=1λAb max. [nm]:
680λEm max. [nm]:
701QY [%]:
30Specs Sheet:
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Description
- Highly hydrophilic, amine-reactive label containing one NHS-ester group.
Applications
- Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
- Fluorescence intensity and fluorescence polarization-based applications
- Resonance Energy Transfer (RET)
- Flow Cytometry
- Immunofluorescence
- Gene Expression
- Homogeneous Assays
- Microarrays
Advantages
- Perfectly suited for excitation with the 635 nm and 670 nm diode lasers
- Sensitive; high extinction coefficients and high quantum yields after covalent attachment to biomolecules
- Quantum yield is highly increased after covalent and non-covalent association with proteins
- pH-insensitive between pH 3 and pH 10
- Good aqueous solubility; this label does not alter the solubility of the bioconjugate
- High photostability; e.g. compared to fluorescein or Cy5.5TM
- Low molecular weight — Seta dyes do not add substantial mass to the conjugates
- Ideal for non-radioactive labeling of proteins, amino-modified oligonucleotides and amino-modified lipids
