Longest single-fluorescent-molecule tracking ever reported in living cells with SeTau-647 - Nature Chem. Biol. (2018)
The photobleaching rates of 13 fluorescent dyes, 4 of them conjugated to trolox, were investigated for single-molecule measurements at 37oC in living cells. Among these SeTau-647 (K9-4149 and K9-4148), a squaraine rotaxane dye, exhibited the best photobleaching performance in this study with an exponential lifetime of 105.8 sec. As a membrane-impermeable dye it allowed to observe up to 12,000 frames, which is the longest single-fluorescent-molecule tracking ever reported. See paper in Nat. Chem. Biol. 14, 497–506 (2018) [32].
| Excitation Light Sources | Characteristics | ||||||||||||||||||||
| Product Number (Specs Sheet) |
Product Name (Product Info) |
Target Group | 380 | 405 | 436 | 488 | 532 | 594 | 635 | 650 | 680 | 700 | 750 | 780 | Medium | λ abs [nm] |
ε [M –1 cm–1] |
λ em [nm] |
QY [%] |
FLT [ns] |
Buy |
| K9-4119 | SeTau-665-NHS | NH2 | • | • | • | PB 7.4 | 664 | 160000 | 712 | 53 | 3.1 | ||||||||||
| K9-4148 | SeTau-647-Maleimide | SH | • | • | PB 7.4 | 648 | 200000 | 692 | 45 | 3.2 | |||||||||||
| K9-4149 | SeTau-647-NHS | NH2 | • | • | PB 7.4 | 649 | 200000 | 695 | 61 | 3.2 | |||||||||||
| K9-4169 | SeTau-670-NHS | NH2 | • | • | • | PB 7.4 | 673 | 275000 | 694 | 36 | 1.6 | ||||||||||
| K9-4179 | SeTau-680-NHS | NH2 | • | • | • | PB 7.4 | 683 | 295000 | 705 | 58 | 2.9 | ||||||||||
