SeTau 647 used in single molecule study of cortical actin nodes

Cortical actin nodes were investigated using super-resolution and single-molecule microscopy techniques with SeTau-647 (K9-4148)-labeled CoA.

 

Movie showing a representative two-color TIRFM observation of Lifeact-mGFP (green) and Lifeact-TM-ACP-SeTau647 (red) at 60 Hz (16.7-ms time resolution) is available here https://doi.org/10.1371/journal.pone.0188778.s007 or online in the citation below.

			Excitation Light Sources												Characteristics
Product Number (Specs Sheet)	Product Name (Product Info)	Target Group	380	405	436	488	532	594	635	650	680	700	750	780	Medium	λ abs [nm]	ε [M –1 cm–1]	λ em [nm]	QY [%]	FLT [ns]	Buy
K9-4119 K9-4119	SeTau-665-NHS SeTau-665-NHS	NH2								•	•	•			PB 7.4	664	160000	712	53	3.1
K9-4148 K9-4148	SeTau-647-Maleimide SeTau-647-Maleimide	SH							•	•					PB 7.4	648	200000	692	45	3.2
K9-4149 K9-4149	SeTau-647-NHS SeTau-647-NHS	NH2							•	•					PB 7.4	649	200000	695	61	3.2
K9-4169 K9-4169	SeTau-670-NHS SeTau-670-NHS	NH2							•	•	•				PB 7.4	673	275000	694	36	1.6
K9-4179 K9-4179	SeTau-680-NHS SeTau-680-NHS	NH2							•	•	•				PB 7.4	683	295000	705	58	2.9

Citation: Shirai YM, Tsunoyama TA, Hiramoto-Yamaki N, Hirosawa KM, Shibata ACE, Kondo K, et al. (2017) Cortical actin nodes: Their dynamics and recruitment of podosomal proteins as revealed by super-resolution and single-molecule microscopy. PLoS ONE 12(11): e0188778. https://doi.org/10.1371/journal.pone.0188778